Thermo Scientific Web Tools

Thermo Scientific separates general molecular-quantity calculators from setup tools for named Phusion polymerases and DyNAmo kits. Its DNA copy-number and dilution guide starts with the DNA's molar mass per bp, an organism selection or a custom fragment length, and the measured stock concentration. Supply the desired copies/µL and final volume to obtain dilution/pipetting volumes. The average base-pair weight can be changed when the DNA requires a different value. Keep those length, molecular-weight and concentration assumptions with the result. Product-specific reaction setups should be used within the named reagent scope; the collection also provides primer, OD-conversion and qPCR-efficiency tools.